Development of a Novel PPARγ Ligand Screening System Using Pinpoint Fluorescence-Probed Protein
نویسندگان
چکیده
منابع مشابه
Development of a novel PPARγ ligand screening system using pinpoint fluorescence-probed protein.
The activation of peroxisome-proliferator-activated receptor-γ (PPARγ), which plays a central role in adipocyte differentiation, depends on ligand-dependent co-activator recruitment. In this study, we developed a novel method of PPARγ ligand screening by measuring the increase in fluorescent polarization accompanied by the interaction of a fluorescent co-activator and PPARγ. Sterol receptor co-...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2011
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.100810